A mechanism of coupling leading and lagging strand DNA synthesis under replication stress in budding yeast

Here we show that the asymmetric DNA synthesis is also observed in mec1-100 and mrc1-AQ cells defective in replication checkpoint, but surprisingly, not in mrc1∆ cells in which both DNA replication and checkpoint functions of Mrc1 are missing. Furthermore, depletion of Mrc1 or its partner in DNA replication, Tof1, suppresses the asymmetric DNA synthesis in rad53-1 mutant cells. ChIP-Seq was performed for Rfa1; BrdU was performed for yeast cells under different conditions.