遇见数据集

Table 2 in Taxonomic revision of Australian Erythrophleum (Fabaceae: Caesalpinioideae) including description of two new species

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Zenodo2025-04-05 更新2026-05-26 收录
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Table 2. Taxa analysed, vouchers, and GenBank and ENA reference numbers. SpeciesVoucherGenBank and ENA accession numbersErythrophleum arenarium R.L.Barrett & M.D.BarrettWestern Australia (WA), Barrett 9080 (NSW, PERTH)MT581272WA, Bean 25079 (BRI)MT581273WA, Byrne 1271 (PERTH 07148453)MT581270WA, Forbes 2465 (PERTH 01958534)MT581269WA, Sweedman 8997 (PERTH 08786607)MT581271Erythrophleum chlorostachysWA, Byrne 3693 (PERTH 08793034)MT581275(F.Muell.) Baill.Northern Territory (NT), Lazarides 8845 (CANB 295340)MT581276WA, Weston 12284 (PERTH 02211750)MT581274NT, Larcombe 2 (DNA D0057605)MT581277NT, Wightman 5205 (DNA D0051920)MT581278Erythrophleum fordii Oliv.China, no voucherITS1 only, consensus assembly from SRR8191117 (Wang et al. 2019) AChina, no voucherContiguous nearly complete (with gaps) 18S–ITS1–5.8S–ITS2–28S sequence, consensus assembly from SRR8191118 (Wang et al. 2019)Erythrophleum ivorense A.Chev.Gabon, Wieringa 5487 (WAG)OQ572325; contiguous complete 18S–ITS1–5.8S–ITS2–28S sequence, consensus assembly from ERR4363217 (Koenen et al. 2020)Erythrophleum pubescens R.L.Barrett & M.D.BarrettWA, Barrett MDB5902 (plant 1) (PERTH)MT581285WA, Barrett MDB5902 (plant 2) (PERTH)MT581286WA, Byrne 3721 (PERTH 08760632)MT581282WA, Coate 224 (PERTH 02888580)MT581279WA, Dauncey H666 (PERTH 08422060) BMT581297WA, Foulkes 340 (PERTH 02523922)MT581280NT, Brennan 4583 (DNA D0146433)MT581290NT, Clark 1670 (DNA D0034313)MT581291NT, Cowie 5303 (DNA D0121980)MT581292NT, Dunlop 7145 (NSW 451601)MT581293NT, Egan 2873 (DNA D0077644)MT581294NT, Evans 3270 (NSW 451600) CMT581289NT, Smith 101 (DNA D0044224)MT581295NT, Smith 128 (DNA D0029224)MT581296NT, Whaite 3979 & Whaite (NSW 415299)MT581283Queensland (Qld), Blake 23184 (PERTH 02211556)MT581281Qld, Leitch QDA003815 (BRI AQ854110)MT581288Qld, McDonald KRM9767 (BRI AQ846978)MT581287Qld, Wannan 213 & Lynch (NSW 396373)MT581284Qld, McDonald KRM17554 (MEL 2416964A)OQ471964 (dominant copy, contiguous complete 18S–ITS1–5.8S–ITS2–28S sequence) andOQ396764 (minor copy, ITS1–5.8S–ITS2 only), consensus assembly from ERR7599610Pachyelasma tessmannii (Harms) HarmsGabon, Wieringa 5229 (WAG)OQ572326; contiguous complete 18S–ITS1–5.8S–ITS2–28S sequence, consensus assembly from ERR4363236 (Koenen et al. 2020) (Continued on next page) A Sample was assembled from short-read archives, but could not be uploaded to GenBank because third-party assemblies require wet-lab experiments to meet requirements. Sequences are provided in the ribosomal alignment (see File S1.nex in the Supplementary material). B The only full-length ITS sequence obtained from Sanger sequencing. C The sample Evans 3270 was excluded from further analyses. Although the sample was resolved with E. pubescens (77% BS in RAxML tree, data not shown) as expected from its morphology, the sequence chromatograms were messy, resulting in ambiguous base calls, and the edited sequence still somewhat divergent from other E. pubescens. Because it was not possible to distinguish among hybridisation, paralogy or contamination as the cause of different base calls to other Erythrophleum haplotypes, the sequence was excluded from analyses. A second sample (Egan 2873) from the same locality (Cutta Cutta Caves, NT) produced a haplotype belonging to the E. pubescens clade, as expected from its morphological features, and it is likely that Evans 3270, likewise, represents E. pubescens; however a hybrid origin for this specimen with E. chlorostachys cannot be confidently excluded.

表2 分析类群、标本凭证以及基因银行(GenBank)与欧洲核苷酸档案馆(ENA)登录号 物种 标本凭证 基因银行(GenBank)与欧洲核苷酸档案馆(ENA)登录号 沙地格木(Erythrophleum arenarium R.L.Barrett & M.D.Barrett) 西澳大利亚州(WA),Barrett 9080(NSW、PERTH) MT581272 西澳大利亚州(WA),Bean 25079(BRI) MT581273 西澳大利亚州(WA),Byrne 1271(PERTH 07148453) MT581270 西澳大利亚州(WA),Forbes 2465(PERTH 01958534) MT581269 西澳大利亚州(WA),Sweedman 8997(PERTH 08786607) MT581271 绿花格木(Erythrophleum chlorostachys (F.Muell.) Baill.) 西澳大利亚州(WA),Byrne 3693(PERTH 08793034) MT581275 北领地(NT),Lazarides 8845(CANB 295340) MT581276 西澳大利亚州(WA),Weston 12284(PERTH 02211750) MT581274 北领地(NT),Larcombe 2(DNA D0057605) MT581277 北领地(NT),Wightman 5205(DNA D0051920) MT581278 福特格木(Erythrophleum fordii Oliv.) 中国,无标本凭证 仅ITS1区域,基于SRR8191117的共识组装序列(Wang等,2019)A 中国,无标本凭证 近完整的连续序列(存在缺口):18S–ITS1–5.8S–ITS2–28S,基于SRR8191118的共识组装序列(Wang等,2019) 象牙海岸格木(Erythrophleum ivorense A.Chev.) 加蓬,Wieringa 5487(WAG) OQ572325;完整连续18S–ITS1–5.8S–ITS2–28S序列,基于ERR4363217的共识组装序列(Koenen等,2020) 柔毛格木(Erythrophleum pubescens R.L.Barrett & M.D.Barrett) 西澳大利亚州(WA),Barrett MDB5902(植株1)(PERTH) MT581285 西澳大利亚州(WA),Barrett MDB5902(植株2)(PERTH) MT581286 西澳大利亚州(WA),Byrne 3721(PERTH 08760632) MT581282 西澳大利亚州(WA),Coate 224(PERTH 02888580) MT581279 西澳大利亚州(WA),Dauncey H666(PERTH 08422060)B MT581297 西澳大利亚州(WA),Foulkes 340(PERTH 02523922) MT581280 北领地(NT),Brennan 4583(DNA D0146433) MT581290 北领地(NT),Clark 1670(DNA D0034313) MT581291 北领地(NT),Cowie 5303(DNA D0121980) MT581292 北领地(NT),Dunlop 7145(NSW 451601) MT581293 北领地(NT),Egan 2873(DNA D0077644) MT581294 北领地(NT),Evans 3270(NSW 451600)C MT581289 北领地(NT),Smith 101(DNA D0044224) MT581295 北领地(NT),Smith 128(DNA D0029224) MT581296 北领地(NT),Whaite 3979 & Whaite(NSW 415299) MT581283 昆士兰州(Qld),Blake 23184(PERTH 02211556) MT581281 昆士兰州(Qld),Leitch QDA003815(BRI AQ854110) MT581288 昆士兰州(Qld),McDonald KRM9767(BRI AQ846978) MT581287 昆士兰州(Qld),Wannan 213 & Lynch(NSW 396373) MT581284 昆士兰州(Qld),McDonald KRM17554(MEL 2416964A) OQ471964(优势拷贝,完整连续18S–ITS1–5.8S–ITS2–28S序列)与OQ396764(次要拷贝,仅ITS1–5.8S–ITS2区域),基于ERR7599610的共识组装序列 塔斯曼厚荚豆(Pachyelasma tessmannii (Harms) Harms) 加蓬,Wieringa 5229(WAG) OQ572326;完整连续18S–ITS1–5.8S–ITS2–28S序列,基于ERR4363236的共识组装序列(Koenen等,2020) (下页续) A 该样本通过短读长存档序列组装获得,但无法上传至基因银行(GenBank),因第三方组装结果需满足湿实验相关要求方可提交。相关序列已包含在核糖体比对文件中(详见补充材料中的File S1.nex)。 B 该样本为通过桑格测序获得的唯一全长ITS序列。 C 样本Evans 3270被排除在后续分析之外。尽管该样本如形态学预期所示,与柔毛格木聚为一支(RAxML系统发育树中自举支持率为77%,数据未展示),但其序列峰图杂乱,导致碱基调用存在歧义,且编辑后的序列与其他柔毛格木序列仍存在一定差异。由于无法明确区分杂交、旁系同源或污染是导致该序列与其他格木属单倍型碱基调用不一致的原因,故该序列被排除在分析之外。来自同一产地(北领地库塔库塔洞穴)的另一份样本Egan 2873,其单倍型属于柔毛格木分支,符合其形态学特征,因此Evans 3270大概率同样属于柔毛格木;但无法排除该标本与绿花格木(Erythrophleum chlorostachys)存在杂交起源的可能性。

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