CIL: 50724, FIB-SEM Dataset of anti-PKHD1L1 Immuno-Gold Labeled Outer Hair Cell Stereocilia Bundles. In Cell Image Library

Imaging mode: DICM Visualization methods: Spinning disc confocal DICM & widefield DICM Using a Nikon TiE microscope equipped with a 40x, 0.95 N.A. objective and a Hamamatsu ORCA-Flash 4.0 V3 Digital CMOS camera, or a Nikon TiE spinning-disc confocal microscope equipped with a Yokogawa CSU-X1 scanning unit, an Andor iXon 888 EMCCD camera, and a 60x, 1.3 N.A. water-immersion objective or a 40x, 0.95 N.A. objective

成像模式：微分干涉对比显微术（Differential Interference Contrast Microscopy，DICM）
可视化方法：转盘共聚焦DICM与宽场DICM
实验采用搭载40倍、数值孔径（Numerical Aperture，N.A.）0.95物镜及滨松ORCA-Flash 4.0 V3数字CMOS相机的尼康TiE显微镜，或采用搭载横河CSU-X1扫描单元、Andor iXon 888电子倍增电荷耦合器件（EMCCD）相机以及60倍、数值孔径1.3水浸物镜或40倍、数值孔径0.95物镜的尼康TiE转盘共聚焦显微镜