Dataset for: The impact of dissolved inorganic nitrogen and phosphorous on responses of microbial plankton to the Texas City “Y” oil spill in Galveston Bay, Texas

A surface water survey and corresponding bioassay experiment was conducted to examine the impact of nutrients on microbial responses to the Texas City “Y” oil spill on March 22, 2015 in Galveston Bay, Texas. On March 25th and March 28th, 2014 surface (1m depth) water samples were collected from 8 stations. Complementary measurements of water temperature and salinity were also taken at each station. Aliquots of collected water were preserved in glass at -20°C for subsequent estimated oil equivalence (EOE), total polycyclic hydrocarbon (PAH), relative PAH, and nutrients, including nitrate (NO3-1), nitrite (NO2-), ammonium (NH4+) and phosphate (Pi), concentration measurements. Nano- and pico-plankton (2-20 um) were enumerated by flow cytometry. Surface water from stations 2 and 3 was used as the initial substrate for bioassay incubations. Water samples were distributed to triplicate pre-autoclaved polycarbonate bottles (1L) and incubated for a 7-day period. Nitrogen (as nitrate) and Pi were added corresponding to standard f/2 medium (https://ncma.bigelow.org/) for nutrient enriched treatments. Incubated water was sampled from each bottle at 0, 1, 2, 3, 4, 5, 6 and 7 days for flow cytometric analysis. Additionally, water was preserved at the end of the 7-day incubation for EOE, total PAH, relative PAH and nutrient measurements as described above. In addition, a meta-analysis documenting historical anthropogenic releases of petrochemicals into Galveston Bay, Texas was conducted. This included a compilation of the total number of spills from 1998-2014 and the total volumes of petrochemicals released by each of the spills.

本研究开展了地表水调查与配套生物测定实验，旨在探究营养盐对2015年3月22日德克萨斯州加尔维斯顿湾德克萨斯城"Y"号漏油事件中微生物响应的影响。分别于2014年3月25日与3月28日，从8个站位采集表层（1米深度）水样。各站位同步补充测定了水温与盐度。将采集的水样分装至玻璃容器中，于-20℃冷藏保存，用于后续测定油当量（estimated oil equivalence, EOE）、总多环芳烃（polycyclic aromatic hydrocarbon, PAH）、相对多环芳烃，以及硝酸盐（NO3⁻）、亚硝酸盐（NO2⁻）、铵盐（NH4⁺）与磷酸盐（Pi）等营养盐的浓度。采用流式细胞术对纳米浮游生物与微微浮游生物（2~20 μm）进行计数。选取站位2与3的表层水样作为生物测定培养的初始基质。将水样均分至3组经高压灭菌处理的1L聚碳酸酯培养瓶中，进行为期7天的培养。针对营养盐富集实验组，添加以硝酸盐形式存在的氮与磷酸盐（Pi），其添加配比符合标准f/2培养基（https://ncma.bigelow.org/）的规范。分别于培养第0、1、2、3、4、5、6、7天从各培养瓶中采集水样，用于流式细胞术分析。在7天培养周期结束时，留存部分水样，用于开展前述的油当量、总多环芳烃、相对多环芳烃及营养盐浓度测定。此外，本研究还开展了一项荟萃分析，系统记录了德克萨斯州加尔维斯顿湾历史上人为排放的石化类污染物事件，其中涵盖1998年至2014年间的溢油事件总数，以及各次溢油事件排放的石化污染物总排放量。