19F-NMR spectra of artificial 17-OPH biosensor (cpOHPFA_1952-20-BLA-253) in apo and holo states

This dataset contains 19F-NMR spectra supporting Figure 3e, Figure 3f, and Supplementary Figure 10 of the manuscript "Artificial allosteric protein switches with machine learning-designed receptors". The data consists of spectra for the cpOHPFA_1952-20-BLA-253 chimera, an artificial allosteric biosensor composed of a machine learning-designed 17alpha hydroxy-progesterone (17-OPH) binding domain inserted into TEM-1 beta-lactamase. To probe conformational dynamics, p-(O)CF3-tyrosine was installed at specific positions (including 105, 229, 230, 256, 389, and 437) via in vivo genetic code expansion.本数据集包含支撑论文《采用机器学习设计受体的人工变构蛋白开关》（"Artificial allosteric protein switches with machine learning-designed receptors"）的图3e、图3f以及补充图10的氟-19核磁共振（19F-NMR）谱图数据。 本数据集涵盖cpOHPFA_1952-20-BLA-253嵌合体的谱图数据：该嵌合体为一类人工变构生物传感器，由插入至TEM-1型β-内酰胺酶中的、经机器学习设计的17α-羟孕酮（17-OPH）结合结构域组装而成。为探究构象动态变化过程，研究人员通过体内遗传密码扩展技术，在指定位点（包括105、229、230、256、389及437位）引入了对(氧三氟甲基)酪氨酸（p-(O)CF3-tyrosine）。