Arbitrary Ca2+-dependent expression data from mouse vascular endothelial cells

Gene expression depending on artbtary Ca2+ elevation in mouse vascular endothelial cells We used microarrays to detail the global programme of gene expression relatd to putative Ca2+- increase. Mouse vascular endothelial cells, F2 (Toda et al, 1990, Cancer Res 50:5526-5530), were transfected with hBACCS2, an optogenetic tool which induces intracellular Ca2+ elevation using blue light (wave length: 470 nm), and blue light was applied on and off for 4 hours. Those cells were then collected, followed by RNA extraction and hybridization on Affymetrix microarrays. A negative control cells did not have hBACCS2.