Fish egg sequences for the Spawning Habitat and Early-Life Linkages to Fisheries (SHELF) project, northern Gulf of Mexico on 2017-07-17

Six commercial vessels and two FIO vessels collected fish eggs on the west Florida Shelf on 2017-07-17. At each station, two types of plankton net tows were conducted to collect eggs for proportional species-composition determination via DNA barcoding, and for estimating the number of eggs under the water surface. DNA barcoding was based on the region of the cytochrome c oxidase 1 gene (COI), which was amplified and sequenced using established methods. Individual eggs were identified using DNA barcoding. Sequences were submitted to NCBI GenBank (https://www.ncbi.nlm.nih.gov/genbank/) under accession numbers MK976037-MK97664.

2017年7月17日，6艘商业作业船与2艘FIO船在西佛罗里达陆架（West Florida Shelf）采集鱼卵。在每个采样站位，均布设两类浮游生物网拖网作业：一类用于通过DNA条形码（DNA barcoding）测定物种组成比例，另一类用于估算水体表层下的鱼卵数量。DNA条形码分析以细胞色素c氧化酶1基因（COI）区域为靶标，采用已建立的实验方法完成基因扩增与测序。通过DNA条形码对单个鱼卵进行物种鉴定。所有测序序列已提交至NCBI基因库（NCBI GenBank，https://www.ncbi.nlm.nih.gov/genbank/），登录号范围为MK976037-MK97664。