Gut microbiota regulate xenobiotic metabolism in the liver

Germ-free (GF) mice can be used as a powerful tool to investigate the role of the intestinal commensal flora in metabolism and immune tolerance. In this study we have used whole genome transcriptome analyses to compare expression levels systemically in liver of germ-free mice and specific pathogen free (SPF) mice. Keywords: Mouse liver tissue germ-free or specific pathogen free vs universal mouse reference Specific Pathogens Excluded from the SPF mice: Virus 1. Mouse Hepatitis Virus (MHV) 2. Mouse Rotavirus (EDIM) 3. Parvoviruses Minute virus of mice (MVM) Mouse parvo virus (MPV) 4. Pneumonia virus of mice (PVM) 5. Sendai virus 6. Theiler's encephalomyelitis virus 7. Ectromelia virus 8. Lymphocytic choriomeningitis virus (LCMV) 9. Mouse adenovirus 10. Mouse cytomegalovirus 11. Reovirus type 3 Bacteria 1. Citrobacter rodentium 2. Clostridium piliforme (Tyzzer’s disease) 3. Corynebacterium kutscheri 4. Mycoplasma pulmonis 5. Pasteurellaceae 6. Salmonella spp. 7. Streptococci-β-haemolytic 8. Streptococcus pneumoniae 9. Helicobacter spp. 10. Streptobacillus moniliformis Parasite 1. Ectoparasites 2. Endoparasites Two-condition experiment, GF mice liver vs. SPF mice liver. Biological replicates: 4 GF mice liver, 4 SPF mice liver. Each replicate was hybridized on 2 arrays with dyeswap. GSMxxx.gpr test is Cy5. GSMxxxdyeswap.gpr test is Cy3.