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Engineering of a high-fidelity Cas12a nuclease variant capable of allele-specific editing

NIAID Data Ecosystem2026-05-01 收录

Cas12a核酸酶

等位基因特异性编辑

数据链接：

https://www.ncbi.nlm.nih.gov/bioproject/PRJNA1055423 数据链接链接失效反馈

官方服务：

资源简介：

The Raw sequence of NGS data for any of the experiments described in the manuscript

应用场景：

创建时间：

2023-12-21

CRISPRsgRNA设计

等位基因特异性编辑

Table S2. AlleleAnalyzer guides for SpCas9 and SaCas9 in BEST1 for optimal coverage of the 1000 genomes cohort, as shown in Fig.Â 3b-e. Guides with â---â as sequence indicate loss of PAM site due

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Additional file 4: of AlleleAnalyzer: a tool for personalized and allele-specific sgRNA design

sgRNA设计

等位基因特异性编辑

Table S4. PAM site annotation example set for the first 100 variants from the 1KGP on chromosome 1. Full dataset is available as denoted in the data availability section. (XLSX 21 kb)

Figshare2019-08-15 更新50

A new Cas12a with TTNA PAM and robust collateral cleavage for nucleic acid detection

CRISPR检测

Cas12a核酸酶

Raw sequencing data of LtCpf1 identification and characterization

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AlleleAnalyzer supporting data

等位基因特异性编辑

sgRNA优化

The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distingu

NIAID Data Ecosystem50

A Cas12a ortholog with stringent PAM recognition followed by low off-target editing rates for genome editing

基因编辑

Cas12a核酸酶

Our study shows that CeCas12a nuclease is active in human cells and the stringency of PAM recognition could be an important factor shaping off-target editing in gene editing. Thus, CeCas12a provides a

NIAID Data Ecosystem40

Engineering of a high-fidelity Cas12a nuclease variant capable of allele-specific editing

资源简介：

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