Whole exome and whole transcriptome sequencing data for human (Homo sapiens) placenta and maternal decidua tissues. Homo sapiens

Term uncomplicated pregnancy tissues. Whole exome and whole transcriptome sequencing data for human (Homo sapiens) placentas from male XY placentas and female XX placentas and maternal decidua tissues. Placental tissue samples were obtained through rapid sampling (30 minutes post-delivery). Tissue samples were taken from the maternal side, midway between the chorionic and basal plates, from the periphery of the lobules. Sampling sites were free of visible infarction, calcification, hematoma and tears (areas of frank visible pathology were avoided). Whenever possible, tissue samples were obtained from distinct cotyledons of the placenta in opposing quadrants of the placenta (far from one another spatially). The sampling protocol was as follows: 1. Orient the placenta maternal side up basal plate uppermost and identify sampling areas in each of the four placental quadrants. 2. At each site, remove the basal plate to remove maternal tissue ~1-2mm by trimming with a pair of sterile scissors to expose villous tissue. 3. Cut a grape-sized approximately 1-2 cm3; 5-6g tissue lobule from each of the four quadrants. 4. Wash the tissue thoroughly twice in phosphate buffered saline PBS solution and blot on clean gauze. 5. From each grape-sized lobule, cut away eight smaller pieces ~1-2 mm3 using a scalpel. RNA: For each sampling quadrant, place four of the tissue pieces in a labeled cryovial containing 1 mL of RNAlater solution RNA stabilizing agent. Store the cryovials in the 4 C benchtop fridge for a minimum of 48 hours, and a maximum of 7 days, per RNAlater manufacturer protocol. After a minimum of 48 hours, use a pipette to remove the RNAlater solution from the cryovials and immediately snap freeze in liquid nitrogen. Store samples at 80 C until ready for use; aliquot individual tissue pieces as needed per research protocols. DNA: For each sampling quadrant, place four of the ~50mg tissue pieces in a labeled cryovial. Snap freeze immediately in liquid nitrogen. Store samples at -80 C until ready for use; aliquot individual tissue pieces as needed per research protocols.