Expansion and improvement of ChinaMu by MuT-seq and chromosome-level assembly of the Mu-starter genome

The sequence-indexed Mutator insertional library, ChinaMu, has been a valuable resource for functional studies of maize genes. Here, we doubled the overall collection using a newly developed Mu-tag isolation method (MuT-seq), and improved the annotation of the identified germinal insertion sites by sequenced reference genome of the Mu-starter.