Table 2 in Biotic elicitors and mechanical damage modulate glucosinolate accumulation by co-ordinated interplay of glucosinolate biosynthesis regulators in polyploid Brassica juncea

Table 2 List of genes/primers used for real-time qRT-PCR assays in the current study. Gene ID/primer nameGene name usedPrimer sequence in 5 0 –3 0BjuB.MYB28.1 FP(RT)MYB28-1ATCTATATATTCGAACAAACGGTBjuB.MYB28.1 RP(RT)CCTATTAACCCTTATTTAACATBjuB.MYB28.2 FP(RT)MYB28-2AAATCCTACTGTCACCACGTCGBjuB.MYB28.2 RP(RT)ATTGCTGGTAGTCTCGGAAACBjuA.MYB28.1 FP(RT)aMYB28-3ATCTTTTTATATTCGAACTGBjuA.MYB28.1 RP(RT)ACATGAGTTCTCGCCTTCTBjuA.MYB28.2 FP(RT) aMYB28-4GAGTCTTCATTACCAAACAGBjuA.MYB28.2 RP(RT)TAAACTCGGGATTACTAACBjuA.MYB28.3 FP(RT) aMYB28-5CGATGGACTAACTACCTAAAACCTGAABjuA.MYB28.3 RP(RT)GTACCCTGTTCAATCAAACGCTTCBra005949 FP(RT)MYB29-1TCTGGAGCAATTTAGTAACAATGCBra005949 RP(RT)CAACCGGGTATGTTTGTCATACBra013000 RP(RT)MYB34-1TCCAAGAATCAAGAAAGTCCATABra035954 RP(RT)AAGTCCAGATCGTCATTCCAABra016553 FP(RT)MYB34-2TCCGTCCACATTCTCTGAGGBra016553 RP(RT)TCGTCGTTCACGTCCTCCTBra025666 FP(RT)MYB51-1TGATTCCTCCGTTAACGATCCTBra025666 RP(RT)CCGAAGAAACCTCGTAAGTTCABra031035 FP(RT)MYB51-2TTAATGTACTGGGATAAGGAAGATGBra031035 RP(RT)CATCATTTCTACGTTGTCCGBra008131 FP(RT)MYB122-GTTCTCTAGCGACACCTCTGACBra008131 RP(RT)1CAATTTTATCGTCTTGAAGAATCCBra015939 FP(RT)MYB122-CTCTAACGATACCTCCGCTCAGBra015939 RP(RT)2GGTGGCTATCATTAAGCGACABjUBQ-9 F(RT)UBQ9GAAGACATGTTCCATTGGCABjUBQ-9 R(RT)ACACCTTAGTCCTAAAAGCCACCT a BjuA.MYB28.1: Homolog of Bra029311; BjuA.MYB28.2: Homolog of Bra0012961 and BjuA.MYB28.3: Homolog of Bra035929.表2 本研究中实时荧光定量逆转录聚合酶链式反应（real-time qRT-PCR）所用基因与引物列表 基因ID/引物名称 | 所用基因名称 | 5′→3′方向引物序列 BjuB.MYB28.1 FP(RT) | MYB28-1 | ATCTATATATTCGAACAAACGGT BjuB.MYB28.1 RP(RT) | — | CCTATTAACCCTTATTTAACAT BjuB.MYB28.2 FP(RT) | MYB28-2 | AAATCCTACTGTCACCACGTCG BjuB.MYB28.2 RP(RT) | — | ATTGCTGGTAGTCTCGGAAAC BjuA.MYB28.1 FP(RT)a | MYB28-3 | ATCTTTTTATATTCGAACTG BjuA.MYB28.1 RP(RT) | — | ACATGAGTTCTCGCCTTCT BjuA.MYB28.2 FP(RT) a | MYB28-4 | GAGTCTTCATTACCAAACAG BjuA.MYB28.2 RP(RT) | — | TAAACTCGGGATTACTAAC BjuA.MYB28.3 FP(RT) a | MYB28-5 | CGATGGACTAACTACCTAAAACCTGAA BjuA.MYB28.3 RP(RT) | — | GTACCCTGTTCAATCAAACGCTTC Bra005949 FP(RT) | MYB29-1 | TCTGGAGCAATTTAGTAACAATGC Bra005949 RP(RT) | — | CAACCGGGTATGTTTGTCATAC Bra013000 RP(RT) | MYB34-1 | TCCAAGAATCAAGAAAGTCCATA Bra035954 RP(RT) | — | AAGTCCAGATCGTCATTCCAA Bra016553 FP(RT) | MYB34-2 | TCCGTCCACATTCTCTGAGG Bra016553 RP(RT) | — | TCGTCGTTCACGTCCTCCT Bra025666 FP(RT) | MYB51-1 | TGATTCCTCCGTTAACGATCCT Bra025666 RP(RT) | — | CCGAAGAAACCTCGTAAGTTC Bra031035 FP(RT) | MYB51-2 | TTAATGTACTGGGATAAGGAAGATG Bra031035 RP(RT) | — | CATCATTTCTACGTTGTCCG Bra008131 FP(RT) | MYB122 | GTTCTCTAGCGACACCTCTGAC Bra008131 RP(RT) | — | CAATTTTATCGTCTTGAAGAATCC Bra015939 FP(RT) | MYB122 | CTCTAACGATACCTCCGCTCAG Bra015939 RP(RT) | — | GGTGGCTATCATTAAGCGACA BjUBQ-9 F(RT) | UBQ9 | GAAGACATGTTCCATTGGCA BjUBQ-9 R(RT) | — | ACACCTTAGTCCTAAAAGCCACCT 注：a BjuA.MYB28.1为Bra029311的同源基因；BjuA.MYB28.2为Bra0012961的同源基因，BjuA.MYB28.3为Bra035929的同源基因。