Analysis on the genetic interaction networks derived from two growth conditions.

(A) Comparison of ratios of alleviating (E-score ≥2) to aggravating (E- score ≤−2) genetic interaction involving essential (E) and non-essential (NE) gene pairs on both growth conditions, p-values were computed using Fisher's exact test. (B) Venn diagram showing the overlap of the genetic interaction pairs derived from this study and the literature. (C) Significance testing of the agreement between the literature and this study (red arrow) compared against expected gene pair frequencies by randomly sampling (blue distribution represents 10,000 null models). (D) Distribution of correlations coefficients between the genetic interaction profiles for gene pairs within the same operon versus randomly drawn gene pairs (panel I). Pair-wise profile correlation coefficient of genes in the Fe-enterobactin “fec” operon in rich medium (panel II) and for LPS 1, 2-glucosyltransferase components of waa operon in minimal medium (panel III). (E) Distribution of genetic interaction profile correlation for gene pairs with correlated co-expression (panel I). Scatter-plot of correlated genetic profiles is shown for cyoD (x-axis) and cyoC (y-axis) in panel II, and for minE (x-axis) and minD (y-axis) in panel III. (F) Distribution of correlation coefficients between the genetic interaction profiles of gene pairs encoding interacting proteins versus randomly drawn gene pairs (panel I), where the p-value was computed using the two-sample Kolmogorov-Smirnov (KS) test. Scatter plot of correlated genetic profiles for mdtI (x-axis) and mdtJ (y-axis) (panel II). Scatter plot of correlated genetic profiles for ftsZ (x-axis) and ftsE (y-axis) (panel III).