Effect of FN14KO on mouse lung immune cells after bleomycin injury

TWEAK, also known as TNFSF12, is a multifunctional cytokine that controls many cellular activities in tissue repair and disease by binding to its receptor Fn14.TWEAK-Fn14 signaling has been shown to induce canonical and noncanonical NFkB signaling, triggering pro-inflammatory responses and leukocyte infiltration.In the bleomycin-induced lung fibrosis model, Fn14 KO mice were found to induce a more severe fibrotic phenotype. To explore the effect of Fn14 KO on immune cell populations in this model, we performed single-cell sequencing. Immune cells from six group of mice(WT saline, FN14KO saline, WT bleomycin D7, FN14KO bleomycin D7, WT bleomycin D14, FN14KO bleomycin D14) were isolated by FACS（CD45+ EPCAM- CD31-）and analyzed using scRNAseq.