Partial characterization of the glycoconjugates of Trypanosoma theileri

Here, we have started to characterise the surface molecules of T. theileri at the biochemical level. We have applied the same differential solvent extraction and hydrophobic interaction chromatography protocols, developed by Almeida and colleagues [13,14] to those used to isolate the GPI-mucin and GIPL fractions of T. theileri. As a positive control, we processed epimastigotes of T. cruzi Silvio X10/7 strain alongside tissue culture forms of T. theileri Edinburgh strain and so also report on the surface molecules of the Silvio X10/7 strain of T. cruzi for the first time.