Rational Design and Construction of Multi-Copy Biomanufacturing Islands in Mammalian Cells

Data underlying the figures in the publication “Rational design and construction of multi-copy biomanufacturing islands in mammalian cells”, published in <em>Nucleic Acids Res.</em>, <strong>2022</strong>, 50(1), 561-578. DOI:10.1093/nar/gkab1214 Table of contents: <strong>1. tableSx.zip: </strong>8 .zip archives containing the raw data for the Tables S1-S8. <strong>2. bioIslands_Supp_Figs_Info_Tables.pdf</strong> Data Availability Statement DNA sequences specifying all coding and regulatory elements used to assemble our multiple gene constructs are provided in the Supplementary Data. Novel sequences have been deposited in the NCBI database. Accession numbers are provided in Supplementary Tables S2-S7. The gene diversifier and optimizer software is freely available for download at https://github.com/altamurr/Gene-Diversifier. Flow cytometry data has been deposited in FlowRepository. Links to FlowRepository are available in the section “Flow Cytometry Data” in the Supplementary Data. Microbial and mammalian strains as well as plasmid constructs used to complete this study are available under a Material Transfer Agreement.本数据集对应发表于《核酸研究》(Nucleic Acids Res.) 2022年第50卷第1期、页码561-578，DOI为10.1093/nar/gkab1214的论文《哺乳动物细胞多拷贝生物制造岛的理性设计与构建》中的配图原始数据。 数据集内容如下： 1. tableSx.zip：内含附表S1至S8原始数据的8个压缩归档文件。 2. bioIslands_Supp_Figs_Info_Tables.pdf：补充图表与信息表文件。 数据可用性声明： 本研究中用于组装多基因构建体的所有编码与调控元件的DNA序列已在补充数据中提供。全新序列已提交至美国国家生物技术信息中心(NCBI)数据库，其登录号详见附表S2至S7。基因多样化优化软件可于https://github.com/altamurr/Gene-Diversifier免费下载。流式细胞术数据已提交至流式细胞术数据存储库(FlowRepository)，相关访问链接详见补充数据中的「流式细胞术数据」章节。本研究所用的微生物与哺乳动物菌株及质粒构建体，需通过材料转移协议获取。