Spatial transcriptomics processed data of normal adrenal cortex

Organism: Homo sapiens

Tissue: Adrenal cortex

Samples: 2 normal adrenal (NAd) samples

Experimental design: FFPE tissue sample was cut into 10-μm section, then processed using the Visium Spatial for FFPE Gene Expression kit (10x Genomics). Section was stained with H&E (Haematoxylin and eosin, Dako) and imaged using a Lamina Slide Scanner (Perkin Elmer). Dual indexed libraries were prepared using the Library Construction Kit with the Dual Index Kit TS Set A (10x Genomics) and sequenced on a NextSeq 500 platform (Illumina) with paired-end sequencing.

Data processing: The demultiplexing, barcoded processing and gene counting were performed with the Space Ranger software v1.3.1.

File format and content: The dataset includes the image files and filtered expression matrices generated by Space Ranger for individual samples. Each filtered expression matrix is saved as 3 files (***_barcodes.tsv.gz, ***_features.tsv.gz, and ***_matrix.mtx.gz) where *** is replaced by the sample name. Image files are saved as 6 files for each sample (***_tissue_hires_image.png, ***_tissue_lowres_image.png, ***_aligned_fiducials.jpg, ***_detected_tissue_image.jpg, ***_scalefactors_json.json, and ***_tissue_positions_list.csv) where *** is replaced by the sample name.