Detection and comparative analysis of cutaneous bacterial communities of farmed and wild Rana dybowskii (Amphibia: Anura)

Rana dybowskii Gunther, 1876 is a dominant amphibian species in northeast China. In order to understand the composition and structure of the cutaneous bacterial communities of farmed and wild R. dybowskii, two experimental groups (farmed and wild) were investigated in this study. Following DNA extraction, the V3-V4 hypervariable region of the 16S rRNA gene was targeted and analyzed by high-throughput sequencing. The cutaneous bacterial community diversity was investigated and compared through analysis of alpha and beta diversity. A total of 524,852 valid sequences and 1,023 operational taxonomic units (OTUs) were obtained from these two experimental groups. The number of shared OTUs was 333, while there were 603 unique OTUs in the farmed group and 87 unique OTUs in the wild group. The Chao, ACE and Shannon indices of the farmed group were significantly higher than those of the wild group (p R. dybowskii exhibited significant differences. This study provides a theoretical basis for comprehensively understanding the composition and abundance of bacteria on the skin of farmed and wild R. dybowskii, which helps to develop its breeding industry and gain economic benefits.

中国林蛙（Rana dybowskii Gunther, 1876）为中国东北地区的优势两栖类物种。为明晰养殖与野生中国林蛙的皮肤细菌群落组成及结构，本研究设置养殖组、野生组两个实验组展开研究。实验首先进行DNA提取，以16S rRNA基因的V3-V4高变区为扩增靶标，通过高通量测序完成分析。通过α多样性与β多样性分析，对皮肤细菌群落多样性开展探究与对比。本研究从两组实验样本中共获得524852条有效序列及1023个操作分类单元（operational taxonomic units，OTUs）。其中共有OTU为333个，养殖组特有OTU 603个，野生组特有OTU 87个。养殖组的Chao指数、ACE指数与香农（Shannon）指数均显著高于野生组（原文此处括号内数据缺失），养殖与野生中国林蛙的皮肤细菌群落组成存在显著差异。本研究可为全面解析养殖与野生中国林蛙皮肤细菌的组成与丰度提供理论依据，有助于推动其养殖产业发展并获取经济收益。