Chromosome-level genome assembly of the autotetraploid yellow pitaya provides novel insights into evolution of trait patterning in pitaya species with different ploidy

Here, we present a high-quality chromosome-level genome assembly of yellow pitaya using PacBio HiFi sequencing and Hi-C scaffolding technologies. We identify yellow pitaya as an autotetraploid with a genome size of 1.79 Gb, harboring 27,246 high-confidence genes probably from diploid ancestors, red pitaya (S. undatus).