Effect of extracellular RNase digestion on cellular RNA from neutrophils and GlycoRNA sequences of neutrophils

These data are used to determine whether extracellular RNase A treatment affects cellular RNA levels. GlycoRNAs were sequenced in both primary bone marrow neutrophils as well as an in vitro neutriphil differentiation cell line (HOXB8). For RNAseq, primary mouse bone marrow neutrophils were harvested, and subjected to extracellular RNase A treatment or mock treatment. Total RNAs were prepared using TRIzol extraction. Two biological replicates were prepared. Libraries were prepared using polyA selection, followed by Illumina sequencing, all performed by Yale Center of Genomic Analysis. For small RNA seq, primary mouse bone marrow neutrophils, HOXB8 cells or differentiated HOXB8 cells were harvested. Total RNAs were prepared using TRIzol extraction. Total RNAs were subjected to glycoRNA purification using either streptavidin beads or WGA beads. Small RNA libraries were prepared, followed by Illumina sequencing by Yale Center of Genomic Analysis.