GWAS pyrethroid-resistant Aedes aegypti

Genome-wide association studies (GWAS) use genetic polymorphism across the genomes of individuals with distinct characteristics to identify genotype-phenotype associations. In mosquitoes, complex traits such as vector competence and insecticide resistance could benefit from GWAS use. We used the Ae. aegypti 50k SNP chip to genotype populations with different levels of pyrethroid resistance from Northern Brazil. Pyrethroids are widely used worldwide to control mosquitoes and other agricultural pests, and their intensive use led to the selection of resistance phenotypes in many insects including mosquitoes. For Ae. aegypti, resistance phenotypes are mainly associated with several mutations in the voltage-gated sodium channel, known as knockdown resistance (kdr). We phenotyped those populations with the WHO insecticide bioassay using deltamethrin impregnated papers, genotyped the kdr alleles using qPCR, and the whole genomic regions with the SNP chip. We identified single-nucleotide polymo...

全基因组关联分析（Genome-wide association studies, GWAS）通过在具有不同特征个体的全基因组范围内检测遗传多态性，以鉴定基因型与表型之间的关联。在蚊子中，媒介效能与杀虫剂抗药性等复杂性状可通过GWAS研究得到深入解析。本研究使用埃及伊蚊（Ae. aegypti）50K单核苷酸多态性芯片（SNP chip），对来自巴西北部、具有不同水平拟除虫菊酯抗性的蚊群进行基因分型。拟除虫菊酯类杀虫剂在全球范围内被广泛用于防治蚊子及其他农业害虫，其大规模使用导致包括蚊子在内的多种昆虫演化出抗药性表型。针对埃及伊蚊，其抗药性表型主要与电压门控钠通道的多个突变相关，这类突变被称为击倒抗性（knockdown resistance, kdr）。本研究通过溴氰菊酯浸渍试纸的世界卫生组织（WHO）杀虫剂生物测定法对上述蚊群进行表型鉴定，采用定量聚合酶链反应（qPCR）对kdr等位基因进行分型，并通过SNP芯片检测全基因组区域的遗传变异。本研究已成功鉴定单核苷酸多态性……