An NMR-based relative binding affinity measurement method has been developed in which differences in the binding affinities of different hosts toward a particular guest (ΔlogKass values) are measured
The excel file contains modeled distributions of Kd values for progressive rounds of aptamer selection and proposed bulk affinity workflow. The origin file includes the construction of gaussi
aData are the average ± standard deviations of three or more independent determinations in general. SPR capturing RON-Fc at 25°C, SPR using immobilized RON Sema/PSI at 25°C, BLI using RON-Fc at 30°C,