RNA-seq analysis of transcriptomes of IL35-Treg cells and the Blimp1-Treg cells

To gain in depth transcriptional information about the two subsets of effector Tregs, we performed RNA-seq analysis to compare the transcriptomes of Thy1.1+Blimp1- (Ebi3-Tregs) and Thy1.1-Blimp1+ (Blimp1-Tregs) Tregs. We found ~173 unique genes with differential expression between the two effector subsets. Overall design: Transcriptioanl profiles of Ebi3-Treg (CD4+CD25+Thy1.1+Blimp1-) and Blimp1-Treg (CD4+CD25+Thy1.1-Blimp1+) isolated from 35EbiT × Blimp1-GFP KI reporter mice were generated by RNA-sequencing.