Antimicrobial susceptibility and virulence-associated genes in Campylobacter isolates from milk and wastewater in Hatay, Turkey

ABSTRACT: Campylobacter is one of the most common causes of bacterial foodborne diseases throughout the world. This study was conducted to determine the prevalence, antimicrobial resistance and virulence of Campylobacter isolates of raw cow’s milk and cattle slaughterhouse wastewater samples in Hatay, Turkey. A total of 114 raw milk and 78 wastewater samples were analyzed for the identification of C. jejuni, C. coli, and C. lari by multiplex PCR. The overall prevalence of Campylobacter was found to be 7.2%, of these isolates, 85.7% were identified as C. jejuni and 14.2% as C. coli, but C. lari was not detected in the study. The cdtA and cadF genes were present in 66.6% and 41.6% of C. jejuni isolates tested, respectively, but wlaN gene was not found in any of the isolates. Results of antimicrobial resistance analysis showed that 71.4% of the isolates were resistant to erythromycin, 64.2% to tetracycline, and 57.1% to ciprofloxacin. Overall, 8 of 14 Campylobacter isolates (57.1%) showed a multidrug resistance.

摘要：弯曲杆菌（Campylobacter）是全球最常见的细菌性食源性疾病致病菌之一。本研究旨在明确土耳其哈塔伊地区生鲜牛乳及肉牛屠宰场废水样本中弯曲杆菌分离株的流行率、抗菌药物耐药性与毒力特征。本研究共收集114份生鲜牛乳样本与78份废水样本，采用多重聚合酶链式反应（multiplex PCR）鉴定空肠弯曲杆菌（C. jejuni）、结肠弯曲杆菌（C. coli）及拉里弯曲杆菌（C. lari）。结果显示，弯曲杆菌总检出流行率为7.2%，其中85.7%的分离株为空肠弯曲杆菌，14.2%为结肠弯曲杆菌，本次研究未检出拉里弯曲杆菌。经检测，66.6%的空肠弯曲杆菌分离株携带cdtA基因，41.6%携带cadF基因，但未在任何分离株中检出wlaN基因。抗菌药物耐药性分析结果表明，71.4%的分离株对红霉素耐药，64.2%对四环素耐药，57.1%对环丙沙星耐药。整体而言，14株弯曲杆菌分离株中有8株（57.1%）呈现多重耐药性。