Reconstruction Of Hippocampus Ca1 Stratum Pyramidale, Ivy Cell

title : Reconstruction of hippocampus CA1 cell morphologies specimen : Rattus norvegicus sex : male weight : 100-200 g This neuron was recorded and filled with biocytin in a 450-500 µm thick coronal slice of adult rat hippocampus, using sharp electrodes (for methods see PMID: 11807843). The fixed slice was resectioned at 50-60 µm and the cell reconstructed with Neurolucida using a 100x oil immersion objective.标题：海马CA1区神经元形态重建 实验标本：褐家鼠（Rattus norvegicus） 性别：雄性 体重：100~200 g 本研究中的神经元取自成年大鼠海马450~500 μm厚的冠状脑片，采用尖锐微电极进行胞内记录并以生物胞素（biocytin）进行标记填充，具体实验方法详见文献PMID: 11807843。固定后的脑片经重新切片至50~60 μm厚度后，使用100倍油浸物镜，通过Neurolucida系统完成该神经元的形态重建。