Multiyear RNA-Seq of Neocalanus flemingeri stages CV and Adult Female from the R/V Tiglax and R/V Sikuliaq in the Northern Gulf of Alaska from 2015-2022

High-throughput sequencing study of field-collected Neocalanus flemingeri pre-adults (stage CV) and adult females between 2015 and 2022. Dataset includes information and accession numbers of the raw sequence reads. Zooplankton collections were made in the northern Gulf of Alaska in collaboration with the Seward Long-term Monitoring Program and the northern Gulf of Alaska Long-term Ecological Research Program (LTER). Pre-adults were collected during the spring from multiple stations, sorted from net collections and immediately preserved. Adult females were collected mostly from Prince William Sound, but also on one occasion from the Gulf of Alaska. Adult females were collected from depth during diapause and preserved upon net retrieval. In addition, time series data were generated in three different years to characterize the post-diapause period through the spawning phase. The purpose of the data collection is to generate gene expression profiles during different years and seasons to evaluate developmental stage and physiological state.