Replication Data for “Deregulating m6A Regulators Leads to Altered RNA Biology in Glioma Cell Lines”

This dataset accompanies the study “Deregulating m6A Regulators Leads to Altered RNA Biology in Glioma Cell Lines”. It examines how perturbation of key m6A RNA regulators—METTL3 (writer), ALKBH5 (eraser), and IGF2BP2 (reader)—affects RNA biology in glioma cell lines. The dataset includes: (1) Nanopore RNA-seq data: Zipped FASTQ files from three biological replicates per condition (Naïve, ALKBH5 knockdown, METTL3 knockdown, IGF2BP2 knockdown, and healthy astrocytes) (2) Gene and transcript abundance estimates: Raw counts and normalized CPM values generated using Bambu Quantification. (3) m6anet outputs: Raw results for each condition, with the replicates pooled. These files represent the source data used for post-sequencing analyses in the study.

本数据集随附于研究论文《m6A调控因子失调导致胶质瘤细胞系RNA生物学特性改变》（Deregulating m6A Regulators Leads to Altered RNA Biology in Glioma Cell Lines）。本研究考察了关键N6-甲基腺苷（m6A）RNA调控因子——METTL3（写入器，writer）、ALKBH5（擦除器，eraser）及IGF2BP2（阅读器，reader）的扰动对胶质瘤细胞系RNA生物学特性的影响。本数据集包含以下内容：
(1) 纳米孔RNA测序（Nanopore RNA-seq）数据：各实验组（未处理组、ALKBH5敲低组、METTL3敲低组、IGF2BP2敲低组及正常星形胶质细胞组）的三个生物学重复样本的压缩FASTQ文件。
(2) 基因与转录本丰度估算结果：通过Bambu定量工具生成的原始计数与归一化CPM（每百万片段计数，Counts Per Million）值。
(3) m6anet分析输出结果：各实验组合并重复样本后的原始分析结果。
上述文件为本研究后续测序后分析所用的源数据。