Transcriptomic analysis of Arabidopsis anac044anac085 and sog1 mutnat under DNA damage condition.

To identify genes whose expression is regulated by SOG1 and ANAC044/085 in response to double strand breaks, wild-type, anac044 anac085, and sog1-1 mutant plants were treated with or without bleomycin for 10 h and were subjected to microarray analysis using Agilent custom microarrays. Five-day-old WT, anac044-1 anac085-1, and sog1-101 plants were transferred to MS medium with or without 0.6 µg/ml bleomycin for 10 h. Total RNAs were extracted from roots, and subjected to microarray analysis. Two independent experiments were performed at each time points using different genotypes for each experiment.