Fine-Tuning of PGC1a Expression Regulates Cardiac Function and Longevity (RNA-seq: telomerase-deficient vs PGC1aOE)

PGC1alpha plays an important role in energy metabolism and mitochondrial function in the heart, multiple attempts have failed to upregulate PGC1alpha expression as a therapy, with the underlying mechanism to be answered. We used RNA sequencing to detail the global programme of gene expression in the hearts of third generation of telomerase-deficient mice with or without cardiomyocyte-restricted PGC1alpha overexpression. Overall design: Heart mRNA profiles of 12-month old telomerase-deficient (G3) and PGC1alpha overexpressed G3 mice were generated by deep sequencing, using Illumina HiSeq.