The integrated single cell RNAseq analysis of lung cells in Alveolar Capillary Dysplasia with Misalignment of Pulmonary Veins (ACDMPV) mouse model

we report the application of single cell RNAseq analysis of lung from Foxf1 S52F heterozygous ACDMPV model, and healthy lung from littermate used as control. Overall design: The mutant sample and control samples were prepared from E18.5 heterozygous mouse embryo. Three embryos were pooled together for both healthy control and mutant sample. The samples were dissociated into small pieces and digested into single cell suspension. The CD45+ cells were depleted by MACS column and CD45- cells used to prepare single cell RNA 3' v3 library for sequencing with 10X Genomics platform.