Kinetics of osmotic stress regulate a cell fate switch of cell survival-Ramp10h -ID-92719-Jurkat cells exposed to hypertonicity

To understand the molecular changes of signaling and markers of cell state when cells are exposed to hypertonic stress, we measured time courses for the below markers: comb ramp10 combined comb ramp10 FSC_H comb ramp10 FSC_W comb ramp10 FSC_A comb ramp10 SSC_H comb ramp10 SSC_W comb ramp10 SSC_A combabe ramp10 BD_IFNy_A700 combabe ramp10 BD_IL1b_PE combabg ramp10 BD_pH2AX_A555 combabg ramp10 BD_FAS_PECy5 combabp ramp10 CS_PP2AC_PE combabo ramp10 BD_Bcl2_PE combabo ramp10 CS_BclXL_A647 combabq ramp10 SC_NOXA_A594 combabq ramp10 SC_ppp2ac_A680
Conclusion:
Conclusions are described in: https://www.biorxiv.org/content/10.1101/2020.07.10.197871v1
Notes:
timepoints 0,48,77,109,134,154,189,268,379,464,535,600 min after addition of 300 mosmol/l NaCl are barcoded by 12 barcodes using Pacific-Blue (dilution: high, mid, low) and Pacific-Orange ((dilution: high, midhigh, midlow, low) following established protocols for fluorescent cell barcoding for flow cytometry: timepoints are barcoded as follows: PO high + PB high=0, PO midhigh + PB high=48, PO midlow + PB high=77, PO low + PB high=109, PO high + PB mid=134, PO midhigh + PB mid=154, PO midlow + PB mid=189, PO low + PB mid=268, PO high + PB low=379, PO midhigh + PB low=464, PO midlow + PB low=535, PO low + PB low=600