Derivation of endometrial gland organoids from term placenta

A limitation of current methods for the generation of endometrial gland organoids is their reliance on decidua isolated from endometrial biopsies or elective abortion. Here we report the establishment of endometrial gland organoids from decidua isolated from term placental membranes. These organoids express typical markers of glandular epithelia such as E-cadherin, Laminin and Cytokeratin 7, and can be propagated in cell culture through multiple passages. Additionally, we identified potential survival factors for the co-culture of organoids and endometrial stromal fibroblasts. These modifications facilitate the generation of patient-specific endometrial gland organoids with known pregnancy outcomes. Overall design: RNA-Seq to determine gene expression profiles of: unprocessed endometrial gland elements (collected from decidua digest), growing organoids derived from those gland elements and endometrial stromal fibroblasts isolated from the same samples and established as primary cell cultures.