Percentage of cells transfected by samples collected from the NGI and assessed by flow cytometry.

RTN suspensions were nebulised through the NGI. Pre-nebulisation samples, the leftover in the nebuliser chamber and the aerosolised samples retrieved from the different NGI stages were used to assess the percentage of cells transfected by flow cytometry. Ctrl = control; PN = suspension prior nebulisation; LO = leftover in the nebuliser reservoir after completion of the nebulisation; Thr = throat; S1–S8 = stages 1–8 of the NGI, S8 = micro-orifice collector. The results of two independent experiments are expressed as mean ± standard error of the mean. For each experiment, duplicate wells were pooled together.