Data from: A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples

A lateral flow immunochromatographic test strip was developed for on-site rapid and sensitive detection of Hexoestrol (HES) residues in fish samples with colloidal gold labeled the anti-HES monoclonal antibody (mAb). The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad, and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 μg/kg, and the limit detection (LOD) value was 0.62 μg/kg. For intra-assay and inter-assay reproducibility, recoveries of HES spiked samples were ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91-4.64% and 4.24-5.17% respectively. High-performance liquid chromatography (HPLC) was employed to confirm the performance of the strip. The strip test only took less than 10 minutes, and thus provides a repaid method for on-site detection of HES residues.

本研究研发出一种侧向流免疫层析试纸条，用于现场快速、灵敏检测鱼类样品中的己烯雌酚（Hexoestrol, HES）残留，该试纸条采用胶体金标记抗HES单克隆抗体（monoclonal antibody, mAb）制备。该试纸条由样品垫、结合物垫、吸水垫以及包含质控线和检测线的检测膜组成。针对鱼类提取样品的检测，该试纸条的灵敏度（半抑制浓度，IC₅₀）经验证为1.86 μg/kg，检测限（LOD）为0.62 μg/kg。在批内和批间重复性评价中，加标HES样品的回收率分别为86.3%~92.3%和85.8%~93.4%，变异系数分别为2.91%~4.64%和4.24%~5.17%。本研究采用高效液相色谱法（HPLC）对该试纸条的检测性能进行了验证。该试纸条单次检测仅需不到10分钟，可为HES残留的现场检测提供一种快速方法。