Supporting data for “Regulation of macrophage polarization and osteoblastic cellular behaviours on titanium surface through the design of surface pattern that facilitates implant-to-bone osteointegration”

The modulatroy role of specific surface topographies on macrophages and osteoblastic cells.Part 1. Modulatory role of Tsurface structures on osteoblasts cellular behaviours.1. surface characterizations2. fluorecent staining and quatification of cells, 3. cell morphologies4. in vitro investigation of osteoblasts cellular behaviours5. in vivo investigation of bone-to-implant integration6. shcematic diagramsPart 2. Regulation of macrophage polarization that facilitates implant-to-bone osteointegration 1. surface characterizations2. macrophage morphologies3. macrophage polarization4. osteogenic differentiation of MSCs5. in vivo investigation of bone-to-implant integration6. transcriptomics ans WB7. schematic diagram

特定表面拓扑结构对巨噬细胞和成骨细胞调节作用的探讨。第一部分：表面结构对成骨细胞细胞行为调节作用的研究。1. 表面特性分析2. 细胞荧光染色及定量3. 细胞形态学研究4. 成骨细胞细胞行为体外研究5. 骨与植入物整合的体内研究6. 绘制示意图。第二部分：调节巨噬细胞极化以促进植入物与骨的骨整合1. 表面特性分析2. 巨噬细胞形态学研究3. 巨噬细胞极化4. 间充质干细胞成骨分化5. 骨与植入物整合的体内研究6. 转录组学和蛋白质印迹分析7. 绘制示意图。