Matlab_Code_Retinalayer_Analysis

The first MATLAB script provides a semi-automated method for measuring **retinal ganglion cell (RGC) layer thickness** and **whole retina thickness** from retinal images. The workflow combines user-guided region-of-interest (ROI) selection with intensity-profile–based landmark identification to enable anatomically informed thickness measurements. The script is intended for research use in retinal imaging studies where manual anatomical verification is preferred over fully automated segmentation. The second MATLAB code performs intensity-based texture analysis of the **core region of the adult zebrafish lens** from OCT-derived or grayscale image data. The primary purpose of the script is to **quantify pixel intensity variability** within the lens core by measuring the **standard deviation (SD)** and the **full width at half maximum (FWHM)** of the pixel intensity distribution. The analysis is semi-automated and relies on minimal user input to ensure consistent anatomical placement of the region of interest (ROI) across samples. If you use this code in your research, please cite the following paper: “Pannexin 1a Regulates Visual System Development and Ocular Integrity in Zebrafish” Shiva Sabour, Sarah Houshangi-Tabrizi, Georg S. O. Zoidl, Georg R. Zoidl, Nima Tabatabaei “iScience( Cell Press) Journal”