Synthesis of negative sense genomic RNA of respiratory syncytial virus

Replication of antigenomic RNA (+ssRNA) of human respiratory syncytial virus (RSV) A, just like replication of genomic RNA, depends on encapsidation by the protein N, which has regions that interact with both protein P and protein L. Encapsidation protects genomic and antigenomic RNA from degradation as these RNAs do not possess the 5' cap and the poly(A) tail. At least 10-times more progeny genome is produced than antigenome, due to a stronger antigenome promoter and due to the genome being used as a template for both transcription and antigenome synthesis (Fearns et al. 2000; Hanley et al. 2010).

人类呼吸道合胞病毒A型（RSV A）的抗原基因组RNA（+ssRNA）的复制，与基因组RNA的复制类似，依赖于蛋白质N的包裹，该蛋白质具有与蛋白质P和蛋白质L相互作用的区域。包裹作用可保护基因组RNA和抗原基因组RNA免受降解，因为这些RNA不含有5'帽子结构和多聚(A)尾巴。由于抗原基因组启动子更为强劲，以及基因组既用作转录模板又用于抗原基因组合成，子代基因组产量至少比抗原基因组高出十倍（Fearns等人，2000；Hanley等人，2010）。