Synthetic lethal interactions.

WT, SA or SE haploid cells were crossed with dyn1Δ, bik1Δ, kar9Δ, bim1Δ, kar3Δ and mad2Δ haploid cells. Crossed diploid cells were sporulated and dissected to assess the viability of resulting haploid double-mutant spores. Double-mutant spores viable and forming a colony was counted as “obtained”, and counts of “obtained” were compared to “predicted” double-mutant spores. Results are: Viable (Yes) when “obtained” was equal or nearly equal to “predicted”; Synthetic Lethal (No) when no “predicted” double-mutant colony was “obtained”, and Synthetic Sick (Sick) when most obtained double-mutant colonies are of small size.