Differential gene expression of human natural killer cells following immobilized IgG1 stimulation

Analysis of human natural killer cells following stimulation with immobilized IgG1 for 4 and 24 hours. Results identified significantly differentially expressed genes in natural killer cells stimulated with immobilized IgG1 providing insight into how antibodies modulate the transcriptome in way that may contribute to cell activation and immune tolerance Natural killer (NK) cells from human peripheral blood mononuclear cells were stimulated for 4 or 24 hours in the presence of absence of immobilized IgG1. Following stimulation, NK cells were collected for total RNA extraction and hybridization to Illumina Human HT-12 v 4.0 Expression BeadChips.