Anguilla japonica Transcriptome or Gene expression

Transcriptomic and iTRAQ proteomic approaches reveal novel short-term hyperosmotic stress responsive proteins in the gill of Japanese eel (Anguilla japonica). Japanese eels weighing 500–600 g were bought from the local fish market and kept in two 40 L glass tanks supplied with charcoal-filtered aerated FW or SW at 18–20 °C under a 12 h light: 12 h dark photoperiod for at least 3 weeks. Gill arches were excised and washed. Gill extracts were collected for RNA or protein extraction.