Single Cell RNA-seq analysis of gene expression patterns of cells from stem-cell-derived-synthetic-blastocysts, called as 'ES-' or 'EPS-blastoids'.

To test the efficacy of stem cell lines to generate 'stem-cell-derived-synthetic blastocysts', we dissociated ES- (built by conventional embryonic stem cells and trophoblast stem cells) or EPS-blastoids (built by extended potential pluripotent stem cells and trophoblast stem cells) into single cells following 96h of culture for single-cell transcriptome analysis. Overall design: Examination of gene expression patterns of cells was performed with the single-cell transcriptome analysis following 96h of culture. Single-cell transcriptome analysis was performed using an inDrops v3 barcoding scheme, and the data preprocessing was performed according to the Github inDrops analysis pipeline in order to generate count matrices (https://github.com/indrops/indrops). The transcriptomic analysis comprises three replicates of SB-EP, two replicates of SB-2iLif and one replicate of natural mouse embryo cells at E4.5.