Synoicum_adareanum_microbiome

The ascidian, S. adareanum, from the Antarctic Peninsula near Anvers Island, is known to produce a bioactive compound, palmerolide A (PalA) that has specific activity to melanoma, a particularly invasive and metastatic form of skin cancer. The combined non-ribosomal peptide-polyketide structure of PalA has similarities to microbially-produced macrolides which motivated this study utilizing culture-dependent and -independent investigations coupled with PalA detection to improve our understanding of the host-associated microbiome and relationship to PalA. Microbiome composition investigations were conducted to describe the microbiome structure and composition of the Antarctic ascidian, Synoicum adareanum. Metadata sets include (1) ascidian sample collections (63 samples) from the Anvers Island archipelago and associated 16S rRNA gene Illumina tag sequence data sets (V3-V4); palmerolide chemistry data was determined for the same set of 63 samples, and is included with the metadata;(2) bacterioplankton sample collections (14 samples) from the Anvers Island archipelago and associated 16S rRNA gene Illumina tag sequence data sets (V3); (3) bacterial isolate 16S rRNA gene sequences (16 isolates, nearly full length 16S RNA gene), cultivated from Synoicum adareanum homogenates.

采自安弗森岛（Anvers Island）附近南极半岛海域的海鞘（ascidian）Synoicum adareanum（缩写S. adareanum）可产生一种具有抗黑色素瘤活性的生物活性化合物——帕尔莫利德A（palmerolide A，PalA），而黑色素瘤是一类侵袭性与转移性极强的皮肤癌亚型。帕尔莫利德A的非核糖体肽-聚酮（non-ribosomal peptide-polyketide）复合结构与微生物来源的大环内酯类（macrolides）化合物结构相似，这一特性促使本研究结合依赖培养与非依赖培养的研究手段，并辅以帕尔莫利德A的检测，以深化对宿主相关微生物组（microbiome）及其与帕尔莫利德A关联的认知。本研究针对南极海鞘Synoicum adareanum的微生物组结构与组成开展了系统分析。本数据集包含如下三类元数据：(1) 采自安弗森岛群岛的海鞘样本（共63份），配套的16S rRNA基因（16S rRNA gene）Illumina标签测序数据集（覆盖V3-V4区域）；同时对该63份样本完成了帕尔莫利德A的化学检测，相关数据亦纳入元数据中；(2) 采自安弗森岛群岛的浮游细菌（bacterioplankton）样本（共14份），配套的16S rRNA基因Illumina标签测序数据集（覆盖V3区域）；(3) 从Synoicum adareanum组织匀浆中分离得到的16株细菌的16S rRNA基因全长序列（接近完整长度）。