Positional cloning of the D-h gene.

(a) Genetic mapping of the D-h locus with STS markers. (b) Fine mapping of the D-h locus with additional STS markers. (c) Candidate gene in the 48 kb genomic DNA region identified by fine mapping. (d) Co-segregation analysis in F2 plants of the HD1×Hwacheongbyeo cross using STS marker S10460. A 583 bp PCR product was observed in the tall homozygotes, whereas a shorter 520 bp PCR product was observed in the dwarf homozygotes. In dwarf heterozygotes, both fragments were observed. Lane 1, Hwacheong; Lane 2, HD1; Lane 3, HD1×HwacheongF1; Lanes 4–19, dwarf phenotype, Lanes 20 to 34, tall phenotype. (e) Genotype of the STS marker S10460 among the HD1 and 33 rice cultivars having normal clum length. Lane 1, HD1; Lanes 2–34, rice cultivars.