A dopamine-induced coordinated gene expression program regulates neuronal function and cocaine response

The goal of this experiment was to determine how dopamine receptor activation in medium spiny neurons alters rapid transcriptional programs. To identify genes altered by dopamine, we used a rat primary striatal neuronal culture system in which striatal neurons were removed from the rat brain at embryonic day 18, cultured for 11 days in vitro using standard neuronal conditions, and treated neurons with either vehicle (neurobasal media) or dopamine (at a concentration of 1µM) for 1hr. This dataset contains PolyA+ RNA-seq results from this experiment, in which we identified 100 upregulated genes and 3 downregulated genes after dopamine stimulation using DESeq2 pipelines. Overall design: RNA-seq 1hr dopamine dataset for "A dopamine-induced coordinated gene expression program regulates neuronal function and cocaine response". This experiment contains 6 biological samples, each of which underwent directional, paired-end PolyA+ RNA-seq on an Illumina HiSeq v4 instrument. Samples were treated with neurobasal media (vehicle; samples S37-1, S37-3, and S37-5), or 1µM dopamine (samples S37-2, S37-4, and S37-6) . Datasets were obtained using RNA-seq from PolyA+ fractions fractions of RNA.