Rule reversal aPCx-to-OB feedback data

While animals readily adjust their behavior to adapt to relevant changes in the environment, the neural pathways enabling these changes remain largely unknown. Here, using multiphoton imaging, we investigated whether feedback from the piriform cortex to the olfactory bulb supports such behavioral flexibility. To this end, we engaged head-fixed mice in a multimodal rule-reversal task guided by olfactory and auditory cues. Both odor and, surprisingly, the sound cues triggered responses in the cortical bulbar feedback axons which preceded the behavioral report. Responses to the same sensory cue were strongly modulated upon changes in stimulus-reward contingency (rule reversals). The re-shaping of individual bouton responses occurred within seconds of the rule-reversal events and was correlated with changes in the behavior. Optogenetic perturbation of cortical feedback within the bulb disrupted the behavioral performance. Our results indicate that the piriform-to-olfactory bulb feedback axons carry stimulus identity and reward contingency signals which are rapidly re-formatted according to changes in the behavioral context.Feedback_responses.zip : All feedback axon boutons imaging data and their associated behavioral informationSniffing.zip : All sniffing data and its associated behavioral informationSuppression.zip : All optogenetic suppression behavioral dataD1_FOV10.zip : All data from the example sessionCode: https://github.com/DiegoEHT/Rule_reversal_aPCx_feedback