Transcriptome profiling of human monocytes from accute phase and convalescent patients infected with Plasmodium vivax malaria

RNA sequencing raw data of monocytes obtained from five patients in acute phase of Plasmodium vivax malaria and 45 days after treatment. Individuals from Rondônia (Brazil) were between 18 and 60 years and attended to the exclusion criteria: chronic inflammatory or infectious diseases, pregnancy, and breastfeeding. Diagnostic of malaria by clinical symptoms and thick blood smear was also confirmed by qPCR. According to the recommended protocol of the Brazilian Ministry of Health patients were treated with chloroquine associated to primaquine. Protocols used were approved by the Ethical Committees on Human Experimentation from Instituto Rene ́ Rachou, Fiocruz, and National Ethical Council (CAAE: 59902816.7.0000.5091). 

          Sequencing was performed in a  NextSeq 500 system using the NextSeq 500/550 High Output Kit v2.5 for 75 cycles (both from Illumina). Before sequencing total mRNA samples were prepared using TruSeq Stranded mRNA Kit (Illumina) according to the manufacturer’s protocol. Files are in FASTQ format, just as they were downloaded from Illumina's cloud environment (BaseSpace) right after sequencing. Files are identified by patient number (1 to 5) and health condition: acute phase of the disease or convalescent (45 days after treatment).