Performance characteristics of temperature-modified ISO 9308-1 method for the enumeration of Escherichia coli in marine and inland bathing waters

Data present the results obtained during establishing of performance characteristics of temperature-modified ISO 9308-1:2014 method. The incubation temperature was changed to 44±0.5°C after the 4-hour resuscitation period at 36±2 °C. Elevated incubation temperature significantly suppressed the growth of thermo-intolerant bacteria and enhanced the selectivity with no negative effect on E. coli recovery. All achieved performance characteristics met the requirements and recommendations set out in ISO 13843:2017 standard. Finally, Chromogenic coliform agar was assessed as a suitable medium for the reliable enumeration of E. coli in bathing water when incubated 17-19 hours at 44±0.5 °C after the 4-5 hours resuscitation period at 36±2 °C.

本数据集呈现了经温度调整的ISO 9308-1:2014标准方法的性能特性构建过程中所获得的实验结果。实验中先在36±2℃下进行4小时复苏培养，随后将培养温度调整为44±0.5℃。升高后的培养温度可显著抑制热敏感细菌的生长并提升方法选择性，且对大肠杆菌（E. coli）的回收率无负面影响。所有测得的性能特性均符合ISO 13843:2017标准中规定的要求与建议。最终评估结果表明，当在36±2℃下复苏4~5小时后，于44±0.5℃下培养17~19小时时，显色大肠菌群琼脂可作为可靠的游泳水域水中大肠杆菌计数的适宜培养基。