Chromomeres represent universal units of chromatin packaging in lampbrush chromosomes. To extend our knowledge on chromomere organization, we applied microdissection to chicken lampbrush chromosomes. In particular, 31 and 5 individual chromomeres were dissected one by one along the macrochromosome 4 and one microchromosome, respectively. The data on genomic context of individual chromomeres was obtained by high-throughput sequencing of the corresponding chromomere DNA.

To extend our knowledge on lampbrush chromomere organization andgenomic context, we performed microdissection of all prominentchromomeres from lampbrush macrochromosome 4 and one of themicrochromosomes in a chicken lampbrush chromosome set. The data ongenomic features of individual chromomeres were obtained byhigh-throughput sequencing. The DNA-library preparation was performedaccording to the manufacturer's recommendations with somemodifications (Ion Torrent, Life Technologies). In particular, primaryDOP-PCR products of the dissected material were re-amplified andbarcoded using a panel of Ion-Torrent primers. The sequencing data wasprocessed and analyzed using the web-based bioinformatic platformGalaxy (https://usegalaxy.org/, Giardine et al., 2005) as earlierdescribed (Zlotina et al., 2016). Analysis of genomic features showedthat the majority of chromomere-loop complexes combine gene-dense andgene-poor regions, while massive loopless DAPI-positive chromomereslack genes and are remarkably enriched with different repetitiveelements.