PGR-B directly binds to promoters of genes important for cell cycle, proliferation, and tumorigenesis within ovarian neoplasia from PGRcre/+mPGRBLsL/+ mice

Long-term expression of PGR-B in the PGRcre/+mPGRBLsL/+ mice results in ovarian neoplasia as early as 23 weeks of age. Microarray analyses in the ovarian tumors exhibit strong molecular signatures of proliferation and tumorigenesis. In order to identify if PGR-B was directly promoting this tumorigenic agenda, ChIP-Seq was performed on ovarian neoplasia from PGRcre/+mPGRBLsL/+ mice at greater than 7.5 months of age. These data suggest PGR-B directly promotes cell cycle related genes, driving the uncontrolled growth in the ovarian tissue. Overall design: A total of five ovarian neoplasia samples were obtained from PGRcre/+mPGRBLsL/+ mice over 7.5 months of age. Tissue was flash frozen and submitted to Active Motif for Factor Path chromatin immunoprecipitation and sequencing analysis.