aApparent kinetic parameters were recorded in 50 mM Tris, 1 mM EDTA, pH 7.5 by varying the concentration of substrate in the presence of 100 µM DCPIP as electron acceptor.n.d., none was detectable.-,
tRNA acylation levels of exponentially growing MG1655 and MG1655relA::HTF measured by northern blotting analysis. Total tRNA was extracted from cells growing in MOPS minimal medium with 0.2% glucose a